β-1,3-Glucanase Inhibits Activity of the Killer Toxin Produced by the Marine-Derived Yeast Williopsis saturnus WC91-2 |
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Authors: | Ying Peng Zhenming Chi Xianghong Wang Jing Li |
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Institution: | (1) Key Laboratory of Marine Genetics and Gene Resource Exploitation (Ministry of Education), Ocean University of China, Yushan Road, No.5, Qingdao, China; |
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Abstract: | The marine-derived Williopsis saturnus WC91-2 was found to produce very high killer toxin activity against the pathogenic yeast Metschnikowia bicuspidata WCY isolated from the diseased crab. It is interesting to observe that the purified β-1,3-glucanase from W. saturnus WC91-2 had no killer toxin activity but could inhibit activity of the WC91-2 toxin produced by the same yeast. In contrast,
the WC91-2 toxin produced had no β-1,3-glucanase activity. We found that the mechanisms of the inhibition may be that the
β-1,3-glucanase competed for binding to β-1,3-glucan on the sensitive yeast cell wall with the WC91-2 toxin, causing decrease
in the amount of the WC91-2 toxin bound to β-1,3-glucan on the sensitive yeast cell wall and the activity of the WC91-2 toxin
against the sensitive yeast cells. In order to make W. saturnus WC91-2 produce high activity of the WC91-2 toxin against the yeast disease in crab, it is necessary to delete the gene encoding
β-1,3-glucanase. |
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Keywords: | |
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