Comparative proteomics analysis of differentially expressed proteins in soybean cell wall during flooding stress |
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Authors: | Setsuko Komatsu Yukito Kobayashi Keito Nishizawa Yohei Nanjo Kiyoshi Furukawa |
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Institution: | (1) National Institute of Crop Science, Kannondai 2-1-18, Tsukuba 305-8518, Japan;(2) Nagaoka University of Technology, Nagaoka 940-2188, Japan |
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Abstract: | Flooding is a major problem for soybean crop as it reduces the growth and grain yield. To investigate the function of the
soybean cell wall in the response to flooding stress, cell wall proteins were analyzed. Cell wall proteins from roots and
hypocotyls of soybeans, which were germinated for 2 days and subjected to 2 days of flooding, were purified, separated by
two-dimensional polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue. Sixteen out of 204 cell wall
proteins showed responses to flooding stress. Of these, two lipoxygenases, four germin-like protein precursors, three stem
28/31 kDa glycoprotein precursors, and one superoxide dismutase Cu–Zn] were downregulated. A copper amine oxidase was found
to have shifted from the basic to acidic zone following flooding stress. Based on these results, it was confirmed by the lignin
staining that the lignification was suppressed in the root of soybean under the flooding stress. These results suggest that
the roots and hypocotyls of soybean caused the suppression of lignification through decrease of these proteins by downregulation
of reactive oxygen species and jasmonate biosynthesis under flooding stress. |
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Keywords: | |
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