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Expression of glycoprotein-processing enzymes in Escherichia coli as fusion proteins |
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| Authors: | Moran DG Yoshida S Fujiyama K Seki T Yoshida T |
| Institution: | (1) International Center of Biotechnology, Osaka University, Yamada-oka 2–1, Suita. Osaka, 565, Japan |
| Abstract: | A truncated Man9-mannosidase gene and a full-length  -1,4-galactosyltransferase gene were isolated from human kidney and placenta cDNAs, respectively. Both genes were cloned in plasmid pMAL-c2 to produce fusions with maltose-binding protein. Fusion products were purified by affinity chromatography. Purified enzymes were assayed using pyridyl-amino labelled oligosaccharides as substrates and analysed by HPLC. |
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