A simple method of analyzing profiles in time-difference direct optical scanning gel chromatography |
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Authors: | John R. Grothusen James K. Zimmerman |
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Affiliation: | Department of Biochemistry, Clemson University, Clemson, SC 29631, U.S.A. |
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Abstract: | A simple method is pressented for the analysis of time-difference, large-zone chromatography profiles, The method is derived from basic theory and is applicable to multicomponent as well as single-component systems. Simple computer simulations are used to demonstrate the inaccuracies of earlier, more empirical methods. This has been tested on several proteins using an inexpensive, semi-automated, data acquisition and control system. |
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Keywords: | Gel chromatography Time difference Large zone Direct optical scanning |
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