| 蛋白质相互作用实验技术的最新进展 |
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| 引用本文： | 王明强,武金霞,张玉红,韩凝,边红武,朱睦元.蛋白质相互作用实验技术的最新进展[J].遗传,2013,35(11):1274-1283. |
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| 作者姓名： | 王明强 武金霞 张玉红 韩凝 边红武 朱睦元 |
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| 作者单位： | 1. 浙江大学生命科学学院遗传学研究所, 杭州 310058;
2. 西藏自治区农牧科学院, 拉萨 850000 |
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| 基金项目： | 国家自然科学基金项目(编号：31171615, 31171543)资助 |
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| 摘 要： | 蛋白质相互作用的研究, 是揭示生物体正常生长发育及其应对各种生物或(和)非生物胁迫的分子机制及其调控网络的重要途径。文章综述了近年来发展起来的研究蛋白质相互作用的常用实验性方法, 如酵母双杂交系统、串联亲和纯化、免疫共沉淀、GST Pull-down、双分子荧光互补、荧光共振能量转移、表面等离子共振分析, 介绍了其原理、发展进程, 并分析了其优缺点。
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| 关 键 词： | 蛋白质相互作用 酵母双杂交系统 串联亲和纯化 免疫共沉淀 GST Pull-down 双分子荧光互补 荧光共振能量转移 表面等离子共振分析 |
| 收稿时间： | 2013-06-05 |
Recent advances in the techniques of protein-protein interaction study |
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| Institution: | 1. Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China;
2. Tibet Academy of Agricultural and Animal Hushandry sciences, Lhasa 850000, China |
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| Abstract: | Protein-protein interactions play key roles in the development of organisms and the response to biotic and abiotic stresses. Several wet-lab methods have been developed to study this challenging area,including yeast two-hybrid system, tandem affinity purification, Co-immunoprecipitation, GST Pull-down, bimolecular fluorescence complementation, fluorescence resonance energy transfer and surface plasmon resonance analysis. In this review, we discuss theoretical principles and relative advantages and disvantages of these techniques,with an emphasis on recent advances to compensate for limitations. |
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| Keywords: | protein-protein interactions yeast two-hybrid system tandem affinity purification Co-immunoprecipitation GST Pull-down bimolecular fluorescence complementation fluorescence resonance energy transfer surface plasmon resonance analysis |
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