Novel mutants of the aubergine gene |
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| Authors: | H Bahar Sahin Omer Faruk Karatas Valeria Specchia Silvia Di Tommaso Céline Diebold |
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| Institution: | 1. IGBMC, CNRS UMR 7104 - Inserm U 964. Illkirch Cedex/ FRANCE;2. Current address: Kadir Has University, Department of Bioinformatics and Genetics, Fatih, ?stanbul/ TURKEY;3. Current address: Department of Molecular Biology and Genetics, Erzurum Technical University, Erzurum/ TURKEY;4. DiSTeBA – Department of Biological and Environmental Science and Technology – University of Salento – Lecce, Italy |
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| Abstract: | Aubergine is an RNA-binding protein of the Piwi clade, functioning in germline in the piRNA pathway that silences transposons and repetitive sequences. Several mutations of this gene exist, but they mostly result in truncated proteins or correspond to mutations that also affect neighboring genes. We have generated complete aubergine knock-out mutants that do not disrupt the neighboring genes. These novel mutants are characterized by PCR and sequencing. Their nature is confirmed by female sterility and by the presence of crystals in testes, common to the aubergine loss of function mutations. These mutants provide novel and more appropriate tools for the study of the piRNA pathway that controls genome stability. |
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| Keywords: | Aubergine argonaute crystal-stellate knockout mutant mutagenesis piRNA sterility |
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