七星瓢虫的卵黄发生:体外培养的脂肪体中卵黄原蛋白的合成

为了深入研究七星瓢虫的卵黄发生及其激素调节机理,我们建立了脂肪体的体外培养方法,证明了脂肪体在体外培养条件下能够正常进行卵黄原蛋白的合成与分泌。在体外合成的卵黄原蛋白与体内合成的具有相同的电泳迁移率、免疫学特性和部分水解肽谱。放射性氨基酸在体外参人卵黄原蛋白的动力学与在体内的相似。用脂肪体的体外培养方法,结合放射免疫沉淀、SAS-聚丙烯酰胺凝胶电泳、放射自显影等技术,研究了不同发育期脂肪体的合成能力,以及取食人工饲料的雌虫中保幼激素类似物对卵黄原蛋白合成的促进作用。

STUDIES ON THE VITELLOGENESIS OF COCC1NELLA SEPTEMPUNCTATA: SYNTHESIS OF VITELLOGENIN BY FAT BODY IN VITRO

In order to further investigate the process of vitellogenesis and the hormonal mechanisms of its regulation in Coccinella septempunctata-an important natural enemy of cotton aphid, we have established the in vitro fat body culture system, which can faithfully reproduce the synthesis and secretion of vitellogenin (Vg) by the fat body in whole insects. The electrophoretic mobilities, antigenicity, and the proteolytic fragmentation patterns of the Vg obtained in vitro were indistinguishable from those of the Vg normally synthesized in vivo. The kinetics o'f incorporation of radioactive amino acid into Vg in vitro was. similar to that in vivo. The synthesis of Vg in the fat body from different stages of ovarian development, and the ZR-512-induced Vg synthesis in the fat body of non-fecund females reared on an artificial diet were examined in the in vitro culture system.