Formation of a chiral acetoinic compound from diacetyl by Escherichia coli expressing meso-2,3-butanediol dehydrogenase |
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| Authors: | S. Ui A. Mimura M. Ohkuma T. Kudo |
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| Affiliation: | Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Japan. |
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| Abstract: | L-Acetoin (L-AC) was produced from diacetyl (DA) by Escherichia coli JM109/pBUD119 containing the meso-2,3-butanediol dehydrogenase (D-AC forming) gene. However, when the strain was cultured in the presence of isopropyl-beta-D-thiogalacto-pyranoside, the enzyme formed catalysed not only D-AC but also L-AC. L-AC was further reduced to L-2,3-butanediol (BD). The yield of L-AC or L-BD from DA (3 gl-1) was about 70% (w/w). |
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