Construction of phylogenetic tree forNicotiana species based on RAPD markers |
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Authors: | Yung-Luen Yu Tsai-Yun Lin |
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Institution: | (1) Department of Life Science, National Tsing Hua University, No. 101, Sec. 2, Kuang Fu Road, 30043 Hsinchu, Taiwan, R.O.C. |
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Abstract: | To apply random amplified polymorphic DNA for analysis of phylogenetic relationships, we used 34 synthetic oligonucleotides
as primers to examine interspecific and intraspecific variations among 18 genotypes, nine species ofNicotiana. The nine species used in this study belong to sectionsTomentosae andAlatae. In addition, we attempted to clarify the taxonomic position ofN. sylvestris. A total of 354 distinct DNA fragments were obtained by polymerase chain reaction. Pair-wise comparisons of unique and shared
amplification products were used to generate Jaccard's similarity coefficients and Nei and Li's similarity coefficients with
the computer software of numerical taxonomy and multivariate analysis system. On the basis of the dendrogram constructed with
the similarity coefficients, the 18Nicotiana genotypes were divided into two clusters. The classification analyzed by RAPD markers is in accordance with the classification
of Goodspeed thatN. sylvestris is a member of sectionAlatae. |
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Keywords: | Nicotiana Phylogenetic tree RAPD |
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