Rat lung glutathione S-transferases: subunit structure and the interrelationship with the liver enzymes |
| |
| Authors: | C A Partridge S V Singh T D Hong C Theodore D D Dao Y C Awasthi |
| |
| Affiliation: | 1. Department of Nephrology, Civil Hospices of Lyon, Lyon Sud Hospital Center, Pierre Benite, France;2. University of Lyon, CarMeN lab, National Institute of Applied Sciences of Lyon (INSA-Lyon), French National Institute of Health and Medical Research (INSERM) U1060, National Institute of Agricultural Research (INRA), Claude Bernard University Lyon 1, Villeurbanne, France;3. Natural and Anthropic Hydrosystems Ecology Laboratory, University of Lyon, Claude Bernard Lyon University 1, National School of Public Works of the State (ENTPE), Villeurbanne, France;4. Association pour l’Utilisation du Rein Artificiel dans la région Lyonnaise (AURAL), Lyon, France;5. Laboratoire d’Analyse Médicale Cerballiance Rhône alpes, Lyon, France;6. Montreal Diabetes Research Center, CRCHUM, Montréal, Quebec, Canada;7. Department of Medicine, University of Montreal, Montreal, Quebec, Canada;1. Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji 611-0011, Japan;2. Research Unit for Physiological Chemistry, The Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Japan;3. Department of Food Science and Nutrition, University of Ulsan, Ulsan 680-749, South Korea;4. Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Suita 565-0871, Japan;1. Department of Plant Agriculture, Gosling Research Institute for Plant Preservation, University of Guelph, Guelph, ON, Canada;2. Department of Horticulture, Faculty of Agriculture, Damanhour University, PO Box 22516, Damanhour, Al-Behira, Egypt;3. Chemistry Department, University of British Columbia, Kelowna, BC, Canada;1. Laboratory of Cell Biochemistry, Department of Biochemistry and Molecular Biology, University of Debrecen, Faculty of Medicine, Debrecen, Hungary;2. MTA-DE Stem Cells, Apoptosis and Genomics Research Group of the Hungarian Academy of Sciences, Debrecen, Hungary;3. Department of Surgery, University of Debrecen, Faculty of Medicine, Debrecen, Hungary;4. MTA-DE Lendület Laboratory of Cellular Metabolism, Debrecen, Hungary;5. Research Center for Molecular Medicine, University of Debrecen, Faculty of Medicine, Debrecen, Hungary;6. Department of Medical Chemistry, University of Debrecen, Faculty of Medicine, Debrecen, Hungary;7. Department of Biophysics and Cell Biology, University of Debrecen, Faculties of Medicine and Pharmacy, Debrecen, Hungary;1. Department of Biochemistry and Molecular Biology, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA;2. Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA;3. Department of Surgery, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA;4. Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA;5. Autophagy, Inflammation and Metabolism Center of Biomedical Research Excellence, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA;6. Department of Metabolism and Endocrinology, Metabolic Syndrome Research Center, Key Laboratory of Diabetes Immunology, Ministry of Education, National Clinical Research Center for Metabolic Diseases, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China;7. Department of Geriatrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China;8. Key Laboratory of Protein Chemistry and Development Biology of State Education Ministry of China, College of Life Science, Hunan Normal University, Changsha, Hunan, China;1. Department of Biochemistry, University of Wisconsin-Madison, Wisconsin, USA;2. Department of Biostatistics and Medical Informatics, University of Wisconsin School of Medicine and Public Health, University of Wisconsin-Madison, Wisconsin, USA;3. Division of Endocrinology, Department of Internal Medicine, Carver College of Medicine, Fraternal Order of Eagles Diabetes Research Center, University of Iowa, Iowa, USA |
| |
| Abstract: | Six forms of glutathione S-transferases designated as GSH S-transferase I (pI 8.8), II (pI 7.2), III (pI 6.8), IV (pI 6.0), V (pI 5.3) and VI (pI 4.8) have been purified from rat lung. GSH S-transferase I (pI 8.8) is a homodimer of Mr 25,000 subunits; GSH S-transferases II (pI 7.2) and VI (pI 4.8) are homodimers of Mr 22,000 subunits; and GSH S-transferases III (pI 6.8), IV (pI 6.0) and V (pI 5.3) are dimers composed of Mr 23,500 and 22,000 subunits. Immunological properties, peptide fragmentation analysis, and substrate specificity data indicate that Mr 22,000, 23,500 and 25,000, are distinct from each other and correspond to Ya, Yb, and Yc subunits, respectively, of rat liver. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
