Electron microscopy of tRNA crystals. II. 4 A resolution diffraction pattern and substantial stability to radiation damage |
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| Authors: | Y Fujiyoshi N Uyeda K Morikawa H Yamagishi |
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| Affiliation: | Institute for Chemical Research Kyoto University, Uji Kyoto-Fu 611, Japan;Department of Biophysics Faculty of Science Kyoto University Kyoto 606, Japan |
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| Abstract: | Electron microscopy was applied to thin crystals of yeast tRNAPhe. The crystals embedded in glucose yield Bragg reflections with a spacing smaller than 4 A. The measurement of radiation damage rate demonstrates that they are 4 to 14 times less susceptible to electron exposures than protein crystals embedded in glucose. |
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| Keywords: | Author to whom correspondence should be addressed. |
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