Scanning Electron Microscopic Studies of Epicuticular Patterns in Mosquito Larvae (Diptera, Culicidae) and Their Use as Taxonomic Characters

The development of integumental patterns on the head capsule in larval instar I-IV of Aedes (Ochlerotatus) cantons (Meigen) was studied by SEM. A comparison of several areas on the head capsule was made. Polygonal patterns of small protuberances (vesicles) were found on the entire head capsule. Arrangements vary between different areas, but within each area they are constant from the II instar throughout larval development. A comparison with five other Aedes spp. in the IV instar shows species specificity of the vesicle patterns at two areas. There the arrangement varies between rhombic and irregular pentagonal shape with sizes from 28 to 42 μm, and from round knobs to more elongated ridges (each from 2.8 μm to 11.2 μm). Treatment with different fixatives was studied. A comparison between saddle and siphon patterns of the six species is made. The possible origin of the vesicles as well as their taxonomic significance is briefly discussed. Species specific patterns exist and improve the possibility of identifying closely similar larvae. Possibly the method when further developed may serve to distinguish species groups.