Energy metabolism of reticulocytes: Two different sources of energy for Na+K+-ATPase activity |
| |
Authors: | Milosav. M. Kosti ,R. V.
ivkovi |
| |
Affiliation: | Milosav. M. Kostić,R. V. Živković |
| |
Abstract: | Total energy production in rabbit reticulocytes amounted to 136·52 ± 6·50μmol ATP h?1ml?1 of reticulocytes: 88·3 per cent was provided by oxidative phosphorylation, whereas only 11·7 per cent by aerobic glycolysis. Na+K+-ATPase accounted for 23 per cent, i.e. 27·65 ± 2·55μmol ATP h?1ml?1 of reticulocytes, in the overall energy consumption in reticulocytes of rabbits. Under basal conditions ATP for Na+K+-ATPase activity was derived exclusively from oxidative phosphorylation. However, when the activity of Na+K+-ATPase was increased due to the stimulation of adenylate cyclase by (?)-isoprenaline, the additional energy required was provided by aerobic glycolysis. These results indicate that two different compartments, one cytosolic and the other mitochondrial, provide energy for Na+K+-ATPase activity in reticulocytes. |
| |
Keywords: | Rabbit reticulocytes energy metabolism Na+K+ATPase (− )-isoprenaline metabolic compartmentation |
|
|