Determination of bone and tissue concentrations of teicoplanin mixed with hydroxyapatite cement to repair cortical defects |
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Authors: | Eggenreich K Zeipper U Schwendenwein E Hadju S Kaltenecker G Laslo I Lang S Roschger P Vecsei V Wintersteiger R |
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Affiliation: | Institute of Pharmaceutical Chemistry and Pharmaceutical Technology, Karl-Franzens University, Schubertstrasse 1, A-8010 Graz, Austria. |
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Abstract: | A highly specific and sensitive isocratic reversed-phase high performance liquid chromatography (HPLC) method for the determination of the major component of teicoplanin in tissue is reported. Comparing fluorescamine and o-phthalaldehyde (OPA) as derivatizing agents, the derivative formed with the latter exhibits superior fluorescence intensity allowing detection of femtomole quantities. Pretreatment for tissue samples is by solid-phase extraction which uses Bakerbond PolarP C(18) cartridges and gives effective clean up from endogenous by-products. Linearity was given from 0.6 to 100 ng per injection. The coefficient of variation did not exceed 5.8% for both interday and intraday assays. It was found that when bone defects are repaired with a hydroxyapatite-teicoplanin mixture, the antibiotic does not degrade, even when it is in the cement for several months. The stability of teicoplanin in bone cement was determined fluorodensitometrically. |
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