| 凝胶色谱法制备寡糖 |
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| 引用本文： | 王维通,腰锐锋,柴文刚.凝胶色谱法制备寡糖[J].中国生物化学与分子生物学报,1986,2(3):69-74. |
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| 作者姓名： | 王维通 腰锐锋 柴文刚 |
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| 作者单位： | 中国科学院化学研究所 北京
(王维通，腰锐锋)，中国科学院化学研究所 北京(柴文刚) |
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| 摘 要： | 采用已知结构的多糖,控制其水解条件,使之产生所需寡糖片断。用聚丙烯酰胺凝胶色谱(BiO-6el P-4)分离,对中性糖可以分离到含十一个糖残基的寡糖。对氨基糖可分离到含七个糖残基的寡糖。用薄层色谱和快原子轰击质谱鉴定了它们的纯度。
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| 关 键 词： | 寡糖 凝胶色谱 |
| 收稿时间： | 1986-06-20 |
PREPARATION OF OLIGOSACCHARiDES BY GEL FILTRATION CHROMATOGRAPHY |
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| Wang,Wei-tong Yao,Rui-feng Chai,Wen-gang.PREPARATION OF OLIGOSACCHARiDES BY GEL FILTRATION CHROMATOGRAPHY[J].Chinese Journal of Biochemistry and Molecular Biology,1986,2(3):69-74. |
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| Authors: | Wang Wei-tong Yao Rui-feng Chai Wen-gang |
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| Institution: | (Institute of Chemistry,Academia Sinica,Beijing |
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| Abstract: | Dextran T70,laminarin,soluble starch,xylan,chitin,and agarose were partially hydrolyzed.The Bio-Gel P-4 columns were used for the separation of some of those hydrolates.Neutral oligosaccharides up to 11 sugar residues and amino-oligosaccharides up to 7 sugar residues were obtained.Thin-layer chromatography and fast atom bombardment mass spectrometry have been used for optimizing hydrolytic conditions and for checking the purities of oligosaccharides obtained. |
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| Keywords: | Oligosaccharides gel Filtration Chromatography |
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