Phospholipase A of Guinea Pig Spermatozoa: Its Preliminary Characterization and Possible Involvement in the Acrosome Reaction |
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| Authors: | KENICHI ONO RYUZO YANAGIMACHI THOMAS T F HUANG JR |
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| Institution: | Department of Anatomy and Reproductive Biology, University of Honolulu School of Medicine, Honolulu, Hawaii 96822 U. S. A. |
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| Abstract: | Phospholipase A activity was demonstrated in guinea pig spermatozoa using U-14C] phosphatidyl choline as a substrate. The activity had a neutral pH optimum, was stimulated by Ca2+ and low concentrations of detergent, and. was inhibited by EDTA, mepacrine and p-bromophenacyl bromide. Appropriate concentrations of mepacrine and p-bromophenacyl bromide inhibited the acrosome reactions of capacitated spermatozoa without interfering with their motility. These results support the notion that phospholipase A is involved in the acrosome reaction of mammalian spermatozoa. |
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