Metabolism of Tritiated Gibberellins in d-5 Dward Maize: II. [H]Gibberellin A(1), [H]Gibberellin A(3), and Related Compounds

After 30 minutes of incubation of young leaf sections of d-5 maize (Zea mays L.) in ³H]gibberellin A₁ (³H]GA₁), the metabolite ³H]GA₈ was present in significant amounts, with a second metabolite, ³H]GA₈-glucose (³H]GA₈-glu), appearing soon after. A third ³H]GA₁ metabolite, the polar uncharacterized conjugate ³H]GA₁-X, took more than 1 hour to appear. The protein synthesis inhibitor cycloheximide inhibited the production of all ³H]GA₁ metabolites, indicating a possible protein synthesis requirement for ³H]GA₁ metabolism.