Macrokinetics and operational stability of immobilized glucose oxidase and catalase |
| |
Authors: | K. Buchholz,B. G delmann |
| |
Affiliation: | K. Buchholz,B. Gödelmann |
| |
Abstract: | Glucose oxidation by immobilized glucose oxidase (GlO) and catalase (Cat) has been investigated in batch and continuous reactions for operational studies. The macrokinetics of the process depend on coupled reaction steps and diffusion rates. The problem may be approximated by a simple pseudohomogeneous model taking into account both substrates of glucose oxidase and the intermediate reaction product H2O2. The effectiveness of both enzymes is enhanced in the coupled reaction path, the overall effectiveness nevertheless is very low. H2O2 causes the inactivation of both GlO and Cat. The rates of deactivation depend on the oxidation rates of glucose that give different quasistationary levels of H2O2 concentration. As a first approximation, the deactivation rates may be described by first-order reactions with respect to H2O2. |
| |
Keywords: | |
|
|