Effects of prophage Mu induction on expression of adjacent host genes |
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Authors: | M S Dubow A I Bukhari |
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Institution: | (1) Cold Spring Harbor Laboratory, P.O. Box 100, 11724 Cold Spring Harbor, New York, USA;(2) Dept.of Microbiology and Immunology, McGill University, 3775 University street, H3A 2B4 Montreal, P.Q., Canada |
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Abstract: | The extent of induction and approximate amount of DNA replication of a Mu prophage carrying a gene for ampicillin resistance can be monitored by assaying the level of -lactamase. The expression of thelacZ gene adjacent to either end of an induced Mu prophage remains virtually unaffected, until late in the Mu lytic cycle, while Mu DNA is replicating and transposing. |
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