Orthophosphate analysis by the Fiske-SubbaRow method and interference by adenosine phosphates and pyrophosphate at variable acid pH |
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| Authors: | B Seddon G H Fynn |
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| Institution: | Department of Biochemistry, University of Manchester, Institute of Science and Technology, Manchester M60 1QD, United Kingdom |
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| Abstract: | ATP, ADP, AMP, and pyrophosphate interfere with colour development in the Fiske-SubbaRow assay procedure. The extent of interference observed is dependent on the pH of the final assay system; small changes in pH at low pH values lead to large variations in the amount of ATP interference. Addition of excess ammonium molybdate and neutralisation of any previous acid additions (such that the pH is maintained constant throughout all assay procedures) removes interference and P1 can be estimated in the presence of relatively high ATP concentrations. |
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