camR, a negative regulator locus of the cytochrome P-450cam hydroxylase operon. |
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| Authors: | H Koga H Aramaki E Yamaguchi K Takeuchi T Horiuchi I C Gunsalus |
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| Abstract: | A 4.27-kilobase insert from a HindIII DNA library of Pseudomonas putida carrying the CAM plasmid allowed coordinate expression of genes camD and camC under control of camR, an upstream regulator. The camC gene specifies cytochrome P-450cam, and camD specifies the 5-exo-alcohol dehydrogenase. A 1.38-kilobase deletion from the insert results in the constitutive expression of genes camC and camD; transformation in trans restores the substrate control, indicating that camR is a negative regulator. |
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