Mutagen formed from tryptophan reacted with sodium nitrite in acidic solution |
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| Authors: | A Ohara M Mizuno G Danno K Kanazawa T Yoshioka M Natake |
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| Affiliation: | Division of Science of Biological Resources, Graduate School of Science and Technology, Kobe University, Japan. |
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| Abstract: | The reaction products from L-tryptophan treated with nitrite under acidic conditions were investigated for mutagenic activity with the Salmonella typhimurium his reversion assay and for DNA-damaging activity using the rec-assay. The diethyl ether extract of the reaction mixture showed 8 spots on thin-layer chromatography (TLC). One compound from the TLC had high mutagenic activity for TA98 without S9 mix, with little DNA-damaging activity. The mutagen was purified and identified by instrumental analysis as 2-hydroxy-(1-N-nitrosoindole)propionic acid (NIHP). The mutagenic activity of NIHP was determined by the induced mutation frequency method; the induced mutation frequency was about 19.2 X 10(-5) at a dose level of 160 micrograms/plate. |
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