高浓度藏红花溶液导致大鼠肝损伤中caspase-3、bcl-2、NF-kB表达及意义

目的：通过研究高浓度藏红花溶液引起肝损伤大鼠肝功能、肝组织病理及肝组织中半胱氨酸蛋白酶-3（caspase-3）,bcl-2,NF-kB表达,探讨半胱氨酸蛋白酶-3（caspase-3）,bcl-2,NF-kB在高浓度藏红花溶液导致的肝损伤中的作用及机制。方法：清洁级雄性大鼠45只,随机分为3组,每组15只,分别标记为A组（藏红花组）;B组（酒精组,阳性对照）;C组（生理盐水组,阴性对照）。分别给予藏红花高浓度水煎浓缩溶液、60%酒精、生理盐水灌胃共6周。第6周末,心腔取血测定ALT、AST。肝组织HE染色,免疫组化方法检测细胞凋亡相关蛋白Caspase-3及调控基因Bcl-2、NF-kB的表达。结果：藏红花组大鼠肝功能ALT、AST升高,肝组织正常结构消失,肝细胞肿胀坏死,部分碎裂,凋亡蛋白caspase-3,bcl-2,NF-kB表达增加（69.6%±16.7%vs 5.3%±1.6%;55.4%±14.5%vs4.5%±2.8%;44.1%±12.6%vs2.5%±1.9%;P〈0.05）。结论：高浓度藏红花溶液可以引起大鼠肝损伤,肝组织caspase-3,bcl-2,NF-kB表达增加,细胞凋亡机制参与了藏红花肝损伤过程。

Expression of Caspase-3, bcl-2 and NF-kB in Hepatic Tissue of Rats with Saffron Induced Hepatic Injury

Objective： To explore the roles of caspase-3, bcl-2 and NF-kB in saffron induced hepatic injury and their machanisms. Methods： A total of 45 male wistar rats were randomly divided into saffron group（n=15）, normal control group（n=15） and model group（alcohol group,n=15）. Rats in the saffron group were fed with saffron 800 mg, kg-1, while rats in the norm al control group and model group were fed with physiological saline and alcohol once a day for 6 month. Blood collection was conducted in all rats at 6 month after last intragastric administration, and levels of serum alanine am inotransferase（ALT） and aspartate aminotransferase（AST） were detected. Caspase-3, bcl-2 and NF-kB assessed by immunohistochemistry,and the apoptotic index of hepatic cells was also measured. Results： Compared to norma1 control group,caspase-3,bcl-2,NF-kB expression in hepatic tissue of rats in saffron group were significantly increased（69.6 % ±16.7 % vs 5.3 % ±1.6 %; 55.4 % ±14.5 % vs4.5 % ±2.8 %; 44.1 % ±12.6 % vs2.5 % ±1.9 %; P0.05）. Conclusions： High dose saffron can induce hepatic injury. The expression of caspase-3, bcl-2 and NF-kB in hepatic tissue of saffron induced hepatic injury increased and may be play an important role in hepatic injury.