甲壳素/海藻酸钠-纳米羟基磷灰石多孔复合支架材料的实验研究(英文)

目的:制备性能优良的复合支架一直是骨组织工程学研究的重点和难点。比较分析甲壳素对复合支架材料的孔隙率、含水量、降解率及生物力学特性的影响。方法:将甲壳素溶液与海藻酸钠溶液充分混合,然后将一定质量的羟基磷灰石加入混合液。根据甲壳素溶液在混合液中的质量分数不同分为两组:sca1(0%chitin)、sca2(50%chitin)。扫描电镜下观察材料的表面结构以及检测材料的孔径。测量并计算出复合支架材料的孔隙率、降解率、含水量以及生物力学性能。结果:两组支架材料均表现为多孔隙结构,平均孔径大小分别为:121.2±12.6μm、213.3±27.3μm。孔隙率分别为:(90.53±1.62)%、(87.73±1.22)%,统计学分析显示,两组材料孔隙率的差异比较有统计学意义(P0.05)。两组支架材料第6周的降解率分别(:59.12±1.93)%、(22.91±0.953)%,统计学分析显示,两组材料降解率的差异比较有统计学意义(P0.05)。两组含水量分别为:(95.52±1.17)%、(90.42±0.85)%,统计学分析显示,两组材料含水量的差异比较有统计学意义(P0.05)。第二组生物力学特性显著提高。结论:从本实验的实验数据可以看出,甲壳素可以增大材料的孔径,提高材料的降解稳定性,提高材料的生物力学强度。因此,甲壳素在骨组织工程领域具有重要的研究价值,同时为今后的进一步实验提供一定的实验依据。关键词:甲壳素;海藻酸钠;纳米羟基磷灰石;复合支架材料;组织工程

Experimental Research of the Novel Macroporous Chitin / Alginate-nanohydroxyapatite Composite Scaffolds*

Objective： It is the emphasis and difficulty to manufacture excellent scaffold for bone tissue engineering research. The purpose of the study was to analyze the effect of chitin to the porosity, water retention, degradation rate and biomechanical characteristics of composite scaffolds. Methods： Chitin solution was mixed with sodium alginate solution, and then the mixture was added a certain quality of hydroxyapatite. We divided the mixture into two groups according to the different chitin solution mass fraction： scal （0 % chitin）, sca2 （50 % chitin）. The surface structure and the pore size was observed under the Scanning electron microscopic. Then we calculated the porosity, degradation rate, water content and biomechanical properties. Results： Two groups of scaffold materials showed a multi-pore structure. The average pore size were 121.2 ± 12.6 μm and 213.3 ±27.3 μm. The porosity were （90.53 ±1.62） % and （87.73 ± 1.22） %. Statistical analysis showed that two groups of material porosity difference were statistically significant （P〈0.05）. The degradation rates of two groups of scaffold materials at six weeks were （59.12 ± 1.93） % and （22.91 ± 0.953） %. Statistical analysis showed that the degradation rate of the material differences between the two groups were statistically significant （P〈0.05）. Water content of two groups of scaffold materials were （95.52 ± 1.17） %and （90.42 ± 0.85） %. Statistical analysis showed that the water content of the material differences between the two groups were statistically significant （P〈0.05）. Biomechanical properties of the second group increased significantly. Conclusion： It could be seen from the experimental data that chitin could increase the pore size, improved stability to degradation and the biomechanical strength of materials. Therefore, chitin may has important research value in bone tissue engineering field.