caspase-2与p53在兔视网膜缺血再灌注损伤中的表达及rh-bFGF对其表达的影响

目的:探讨兔视网膜缺血再灌注损伤(RIRI)中caspase-2、P53蛋白表达及重组人碱性成纤维细胞生长因子(Recombinant human basic fibroblast growth factor,rh-bFGF)对其影响。方法:将104只健康纯种大耳白兔随机分为正常组、缺血再灌注模型组、rh-bFGF治疗组,各组均将左眼作为实验眼。缺血再灌注模型组和rh-bFGF治疗组按照不同再灌注时间各分为1h、6h、12h、24h、48h、72h 6个时间段。后两组兔双眼做缺血再灌注损伤模型后,缺血再灌注模型组给予平衡盐溶液,rh-bFGF治疗组给予rh-bFGF药物治疗。免疫组织化学法检测视网膜组织中caspase-2、P53蛋白的表达变化。结果:caspase-2、P53在正常视网膜组织中几乎不表达,在缺血再灌注1h开始表达,24h达到高峰,48h开始减弱,后逐渐下降,rh-bFGF治疗组各时间点观察指标变化趋势与缺血再灌注模型组基本相似。rh-bFGF治疗组与模型组比较,两种蛋白表达均明显减弱,再灌注6-72h各时段差异有显著统计学意义(P0.05)。结论:rh-bFGF通过抑制视网膜缺血再灌注时caspase-2、P53基因的表达减少视网膜细胞的凋亡,保护视网膜组织。

The Expression of Caspase-2 and p53 in Rabbit Retina inIschemia-Reperfusion Injury and after Rh-bFGF Treatment*

Objective： To investigate The expression of caspase-2 and P53 in rabbit retina in ischemia-reperfusion injury and after rh- bFGF treatment. Methods： One hundred and four healthy adult rabbits without eye diseases were randomly divided into normal contral group,ischemia-reperfusion model group and rh-bFGF treatment group, lschemia-reperfusion model group and rh-bFGF treatment group were subdivided into lhours, 6 hours, 12 hours, 24 hours, 48 hours and 72 hours after reperfusion groups.In ischemia-reperfusion model group and rb-bFGF treatment group, the dual eyes estabilished the ischemia-reperfusion injury models, saline was given to rabbit in ischemia-reperfusion model group and rh-bFGF drug therapy performed in rh-bFGF treatment group.Immunohistochemical method was used to measure changes of caspase-2, P53 protein levels in retinal tissues. Results： No expression of caspase-2, P53 positive cells were found in normal contral group. At the ischemia-reperfusion model group, the expression of caspase-2, P53 began to increase at 1 hours after reperfusion. At 24 hours aider reperfusion the expression reached the peak, kept expressing strongly at the 48th hour, and decreased at the 72nd hour. The treatment groups of rh-bFGF had the same trend with the ischemia-reperfusion model group in each index.rh-bFGF treatment group being compared with ischemia-reperfusion model group, caspase-2,P53 expression was significantly reduced.There was statistical significance of the expression of caspase-2, P53 between the ischemia-reperfusion model group and rh-bFGF treatment group 6-72 hours after reperfusion （P〈0.05）. Conclusion： Caspase-2,P53 protein had taken part in the RIRI.rh-bFGF could cut the expression of caspase-2, P53 protein,and protect the retina tissue.