光照对淫羊藿活性成分生物合成的影响

淫羊藿作为传统的中药材,具有补肾阳、强筋骨、祛风湿等功效.其主要功能活性成分为黄酮醇苷成分淫羊藿苷、朝藿定A、B、C等.本实验在武汉植物园内的药园和园外园两个不同生长环境下,研究两种不同光照对巫山淫羊藿(Epimedium wushanense T.S.Ying)WY品系和箭叶淫羊藿(Epimedium sagittatum( Sieb.& Zucc.)Maxim.) LT1、HN3品系4种主要活性成分生物合成的影响.结果显示,在药园环境生长的淫羊藿的光合速率要高于园外园生长的淫羊藿,药园生长的箭叶淫羊藿LT1、HN3品系的4种活性成分含量均高于园外园生长的同品系,而巫山淫羊藿WY品系则相反.光合速率对4种活性成分含量的积累有一定影响,与朝藿定A、B和淫羊藿苷含量呈显著正相关,与朝藿定C含量呈显著负相关.文中对光合速率与活性成分积累间的关系进行了讨论,还针对不同的淫羊藿品系提出了相应的栽培建议.

Impact of Light Intensity on Biosynthesis of Active Ingredients in Epimedium

As a traditional Chinese medicinal herb, epimedii has effects on toning kidney, strengthening bones and muscles,and treating rheumatism. Its bioactive ingredients are mainly prenylated flavonol glycosides, including icariin, epimedin A, B, and C. Two light conditions E Yao-yuan （Medicinal Herb Garden） of Wuhan Botanical Garden, Yuan-wai-yuan of Wuhan Botanical Garden] were used to study how light affected the biosynthesis of the major bioactive ingredients in Epimedium wushanense T. S. Ying and Epimedium sagittatum （Sieb. ＆ Zucc. ） Maxim. Results showed that the photosynthesis rate was higher in Yao-yuan than in Yuan-wai-yuan for both species. The four bioactive ingredients of E. sagittatum were higher in Yao-yuan than in Yuan-wai-yuan, but the opposite was found for E. wushanense. Photosynthesis rate had significant positive correlation with the content of epimedin A, B and icariin, but had significant negative correlation with epimedin C. The relationship between photosynthesis rate and accumulation of bioactive ingredients was discussed. Suggestions were also proposed for cultivating different Epimedium species.