The dried corncob as a source of DNA for PCR analysis期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

The dried corncob as a source of DNA for PCR analysis

Authors:	M.?C.?Schneerman author-information" > author-information__contact u-icon-before" > mailto:schneerm@uiuc.edu" title=" schneerm@uiuc.edu" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,J.?Mwangi,B.?Hobart,J.?Arbuckle,D.?A.?Vaske,J.?C.?Register,D.?F.?Weber

Affiliation:	(1) Department of Crop Sciences, University of Illinois, 61801 Urbana, IL;(2) Illinois State University, 61790 Normal, Department of Biological Sciences, IL;(3) Pioneer Hi-Bred International, Inc., PO Box 1004, 50131-1004 Johnston, IA;(4) Sygenta, Stanton, MN

Abstract:	We developed a procedure to isolate DNA from dried corncobs. This DNA was amplified successfully by PCR, producing well-defined bands in response to specific primers. The SSR patterns between cob and leaf DNA of the same inbred line were found to be identical, indicating that the DNA in the cob cells had not degraded during senescence.

Keywords:	corncob DNA PCR
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