Detection of Shiga-toxigenic Escherichia coli (STEC) in fresh seafood and meat marketed in Mangalore, India by PCR |
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| Authors: | H Sanath Kumar SK Otta I Karunasagar & I Karunasagar |
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| Institution: | Department of Fishery Microbiology, College of Fisheries, University of Agricultural Sciences, Mangalore-575 002, India. |
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| Abstract: | AIMS: To study the incidence of Shiga-toxigenic Escherichia coli (STEC) in seafoods from India. METHODS AND RESULTS: Escherichia coli isolated from various seafoods such as fresh fish, clams and water were screened for the presence of stx, hlyA and rfbO157 genes by PCR; 5% of clams and 3% of fresh fish samples were positive for non-O157 STEC. CONCLUSIONS: STEC is prevalent in seafoods in India, and non-O157 serotype is more common. SIGNIFICANCE AND IMPACT OF THE STUDY: Seafood could be a vehicle for transmission of STEC even in tropical countries. |
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