Overexpression of Periostin in Stroma Positively Associated with Aggressive Prostate Cancer |
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| Authors: | Yuan Tian Caitlin H Choi Qing Kay Li Farah B Rahmatpanah Xin Chen Sara Ruth Kim Robert Veltri David Chia Zhen Zhang Dan Mercola Hui Zhang |
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| Institution: | 1. Department of Pathology, Johns Hopkins University, Baltimore, Maryland, United States of America.; 2. Department of Pathology, University of California at Irvine, Irvine, California, United States of America.; 3. Department of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, California, United States of America.; Weizmann Institute of Science, ISRAEL, |
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| Abstract: | BackgroundPeriostin is an important extracellular matrix protein involved in cell development and adhesion. Previously, we identified periostin to be up-regulated in aggressive prostate cancer (CaP) using quantitative glycoproteomics and mass spectrometry. The expression of periostin was further evaluated in primary radical prostatectomy (RP) prostate tumors and adjacent non-tumorous prostate tissues using immunohistochemistry (IHC). Our IHC results revealed a low background periostin levels in the adjacent non-tumorous prostate tissues, but overexpressed periostin levels in the peritumoral stroma of primary CaP tumors.MethodsIn this study, periostin expression in CaP was further examined on multiple tissue microarrays (TMAs), which were conducted in four laboratories. To achieve consistent staining, all TMAs were stained with same protocol and scored by same image computation tool to determine the total periostin staining intensities. The TMAs were further scored by pathologists to characterize the stromal staining and epithelial staining.ResultsThe periostin staining was observed mainly in peritumoral stromal cells and in some cases in tumor epithelial cells though the stronger staining was found in peritumoral stromal cells. Both periostin stromal staining and epithelial staining can differentiate BPH from CaP including low grade CaP (Gleason score ≤6), with significant p-value of 2.2e-16 and 0.001, respectively. Periostin epithelial staining differentiated PIN from low grade CaP (Gleason score ≤6) (p=0.001), while periostin stromal staining differentiated low grade Cap (Gleason score ≤6) from high grade Cap (Gleason score ≤6) (p=1.7e-05). In addition, a positive correlation between total periostin staining and Gleason score was observed (r=0.87, p=0.002).ConclusionsThe results showed that periostin staining was positively correlated with increasing Gleason score and the aggressiveness of prostate disease. |
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