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Rat immunoglobulin delta heavy chain gene: nucleotide sequence derived from cloned cDNA |
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| Authors: | Jos phine Sire, Charles Auffray,Bertrand R. Jordan |
| Affiliation: | A Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Case 906—13288, Marseille cédex 9, France a Unité de Génétique et Biochimie du Développement, ERA CNRS 851 — Institut Pasteur, 25, rue du Dr. Roux 75724, Paris cédex 15, France |
| Abstract: | Rat immunoglobulin δ heavy-chain mRNA has been isolated. RNA blot analysis revealed that this mRNA with a length of 1.8 kb encodes for the secreted form of IgD. The corresponding cDNA was cloned in plasmid pBR322 and its sequence was determined. The hybrid plasmid contains a 775-bp insert comprising a partial Cδ1 sequence and complete CδH, Cδ3, CδDC and 3' untranslated sequences. Rat and mouse IgD amino acid sequences show striking homology in Cδ3 and CδDC regions. |
| Keywords: | Recombinant DNA RNA gel blotting IgD myeloma hybrid pBR322 plasmid amino acid sequence homologies |
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