饮酒小鼠动物模型建立及其对雌性激素的影响

目的建立模拟人类饮酒的小鼠动物模型,并以此动物模型进一步研究酒精对小鼠雌激素水平及乳腺癌的影响。方法 SPF级C57BL/6雌性小鼠,随机分对照组和酒精组两组,酒精组20:00到次日8:00给予含有一定浓度酒精的饮用水,其他时间给予常规饮用水,对照组全天给予常规饮用水。观察两组小鼠的饮水量及体重变化;用ANALOX AM1酒精分析仪检测小鼠凌晨2∶00和8∶00血液的酒精浓度(BEC);酶联免疫法(ELASA)检测两组小鼠血清中雌激素水平的差异。结果饮酒组小鼠血液BEC明显增高,类似人类饮酒水平,饮酒组小鼠的饮水量及体重无明显变化;饮酒组小鼠体内雌激素的水平明显高于对照组。结论成功的建立模拟人类饮酒的动物模型,并通过此动物模型初步证实酒精刺激可以增加小鼠体内血清雌激素的水平。

Establishment of a mouse model of alcohol drinking and the effect of ethanol on estrogen in mice

Objective A mouse model of ethanol consumption was set up.This model was used to investigate the effect of ethanol-exposed on the serum estrogen level and breast cancer progression in mice.Methods 2% ethanol in drinking water was given in the ethanol-exposure group mice（n=15） from 8：00 pm to 8：00 am and then replaced with regular water without ethanol at the remaining 12 hours each day for 3 weeks.Mice in the control group（n=15） were provided with regular drinking water only.The water intake,body weight and blood ethanol concentration（BEC） of the mice were checked.The estrogen level in mice serum was quantified by enzyme-linked immunosorbent assay（ELASA）.Results The BEC in mice was much higher than that in control mice and similar to drinking humans.No water intake,body weight differences were found in mice with ethanol exposure or without.The serum estrogen level in ethanol drinking group was much higher than that in the control group.Conclusion A mouse alcohol-exposure model has been successfully established.Ethanol consumption might increase the serum estrogen level in mouse.