Molecular cloning of KLRI1 and KLRI2, a novel pair of lectin-like natural killer-cell receptors with opposing signalling motifs |
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| Authors: | Per C Saether Ingunn H Westgaard Line M Flornes Sigurd E Hoelsbrekken James C Ryan Sigbjørn Fossum Erik Dissen |
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| Institution: | (1) Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway;(2) Veterans Administration Medical Center, Northern California Institute for Research and Education, University of California, San Francisco, CA 94121, USA |
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| Abstract: | We here report the molecular cloning of a novel family of killer-cell lectin-like (KLR) receptors in the rat and the mouse, termed KLRI. In both species, there are two members, KLRI1 and KLRI2. While the extracellular lectin-like domains of KLRI1 and KLRI2 are similar 74% (rat) and 83% (mouse) amino acid identity], they differ intracellularly. KLRI1 has two immunoreceptor tyrosine-based inhibition motifs (ITIMs) in the cytoplasmic domain, suggesting an inhibitory function. KLRI2 has no ITIM, but a positively charged lysine residue in the transmembrane region, suggesting association with activating adapter molecules. Klri1 and Klri2 are localized within the natural killer (NK) cell gene complex on rat chromosome 4 and mouse chromosome 6. By RT-PCR and Northern blot analysis KLRI1 and KLRI2 were selectively expressed by NK cells in both rat and mouse. Epitope-tagged expression constructs of rat KLRI1 and rat KLRI2 induced surface expression of a nondisulphide-linked protein of Mr 36,000/39,000 and Mr 34,000, respectively. |
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| Keywords: | Natural killer cells Receptors Signalling motifs |
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