Localisation of GYIRFamide immunoreactivity inMacrostomum hystricinum marinum (Plathelminthes,Macrostomida) |
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| Authors: | G R Mair R N Johnston D W Halton C Shaw C F Johnston D Reiter R M Rieger |
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| Institution: | (1) School of Biology and Biochemistry, Medical Biology Centre, The Queen's University of Belfast, 97 Lisburn Road, BT69 7BL Belfast, Northern Ireland, UK;(2) Comparative Neuroendocrinology Research Group, The Queen's University of Belfast, BT7 1NN Belfast, Northern Ireland, UK;(3) Department of Zoology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria |
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| Abstract: | The distribution of GYIRFamide immunoreactivity in the nervous system ofMacrostomum hystricinum marinum has been demonstrated by an indirect fluorescence technique in conjunction with confocal scanning laser microscopy (CSLM).
Immunostaining was extensive in both the central (CNS) and peripheral (PNS) nervous systems, revealing detailed information
on the microanatomy of the peptidergic nervous system of this free-living plathelminth. In the CNS, immunoreactive nerve cell
bodies and nerve fibres occurred in the brain and along two pairs of longitudinal nerve cords: the main nerve cords and the
ventral nerve cords. In the PNS, immunostaining was prevalent in nerve cells and fibres innervating the pharynx and the gut.
The employed antibody is directed against a recently characterised FMRF-amide-related peptide (FaRP), GYIRFamide, isolated
from two species of the Tricladida,Dugesia tigrina andBdelloura candida. Phylogenetically, GYIRFamide represents the most ancient neuropeptide thus far identified within the Bilateria |
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