RAPD analysis of Aeromonas salmonicida and Aeromonas hydrophila |
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| Authors: | M Miyata T Aoki V Inglis T Yoshida M Endo |
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| Institution: | Department of Aquatic Biosciences, Tokyo University of Fisheries, Minato, Tokyo, Japan, 1Institute of Aquaculture, University of Stirling, Stirling, UK, and 2Miyazaki University, Department of Animal, Grassland and Fisheries Sciences, Faculty of Agriculture, Miyazaki, Japan |
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| Abstract: | The randomly amplified polymorphic DNA (RAPD) technique was used to analyse the genetic differentiation of 13 strains of Aeromonas salmonicida subsp. salmonicida , and seven strains of Aer. hydrophila. Reproducible profiles of genomic DNA fingerprints were generated by polymerase chain reaction (PCR) using a single randomly designed primer. The RAPD profiles of all the non-motile aeromonads, Aer. salmonicida subsp. salmonicida were identical. However, profiles of the motile aeromonads, Aer. hydrophila differed between isolates. These findings reveal genomic homogeneity in Aer. salmonicida subsp. salmonicida and genetic variety in Aer. hydrophila strains. |
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