羊输卵管液中发情相关糖蛋白的性质研究

哺乳动物输卵管液为受精和早期受精卵的发育提供了一个理想的生理生化环境。人们对输卵管液的成分已进行了初步研究，其中发情相关糖蛋白（ＥＧＰ）是被研究的成分之一。ＥＧＰ仅存在在排卵核受精前后的输卵管液中。当受精卵进入子宫区，输卵管液中的ＥＧＰ就消失了。显然，ＥＧＰ是与哺乳动物的早期发育过程密切相关的。尽管人们对ＥＧＰ的确切生理功能尚不清楚，但作为第一步，首先弄清ＥＧＰ的理化性质是十分重要的。本文目的在于研究羊输卵管液中ＥＧＰ的理化性质。本文采用单向（１Ｄ）、双向（２Ｄ）ＳＤＳ－聚丙烯胺凝胶电泳（ＰＡＧＥ）和等电聚胶电泳（ＩＥＦ），结合Ｗｃｓｔｅｒｎｂｌｏｔ技术对ＥＧＰ的性质进行了研究。由于单克隆抗体及花生凝集素（ＰｅａｎｕｔＡｇｇｌｕｔｉｎｉｎ）和ＥＧＰ相结合的专一性很高，所以不必事先对ＥＧＰ进行纯化就可直接进行分析，我们的结果证明羊ＥＧＰ包括两种蛋白质，一种是酸性蛋白，它的等电点为数４．５；另一种是碱性蛋白，其等电点是８．０；这两种蛋白亚基的分子量相同，都是１０ＫＤ。从不同物种间ＥＧＰ这些理化数据上比较，羊和狒狒的非常接近，但和其他动物的有很多不同。我们认为来自不同动物的ＥＧＰ可能是一类性质相同的蛋白质，具有相

Characterization of an Estrus-Associated Glycoprotein in Oviductal Fluid of Sheep

Estrus-associated glycoprotein (EGP) is a special protein in oviductal fluid which appears before ovulation and fertilization and disappears after the embryo passes into the uterus. One-and two dimensional SDS-PAGE and Western blotting with monoclonal antibody or peanut agglutinin were used to characterize the glycoprotein,The specificity of the marking agents enabled the study to proceed without purification. EGP was found to include two proteins: one is acidic with a pH of 4.5 while the other is basic with a pH of 8.0 The molecular weights of their subunits are the same, that is 110 kD. This study may shed light on the mechanism of reproductive Process and be helpful to the development of a more cffective culture medium for in vitro culture of early embryo.