A simple approach for discovering common nonelectrophoretic enzyme variability: A heat denaturation study in Drosophila melanogaster

A simple procedure is described to detect genetic heterogeneity within electrophoretic classes at a locus in Drosophila, based on electrophoresis and heat denaturation studies. Temperature-resistant (tr) and temperature-sensitive (ts) isoelectrophoretic alleles at the phosphoglucomutase locus (Pgm) are present at polymorphic frequencies in natural and in laboratory populations of Drosophila melanogaster.This work has been supported by a grant from the Consiglio Nazionale delle Ricerche (C.N.R.).