Oligo- and polynucleotide probes. A molecular hybridization method

In this review the main principles and possibilities of a hybridization analyses as a highly specific methods for diagnostics of infections and genetic diseases are discussed. Special attention is paid to the chemical approaches to the preparation of non-radioactive probes based on synthetic oligonucleotides and polynucleotides containing biotin, Eu3+ and enzyme labels. Some versions of the hybridization analysis technique are discussed.