a Istituto di Chimica, Universita degli Studi di Trieste, 34127, Trieste, Italy
* Biophysics Laboratories, Portsmouth Polytechnic, Portsmouth PO1 2DT, Hants, UK
Abstract:
Using formaldehyde as a reversible crosslinker, it has been shown that an heterologous dimer is the principal components in a mixture of calf thymus H2A and sea urchin H2B1. Comparison of the crosslinking ability of formaldehyde with dimethyl suberimidate demonstrates that suberimidate gives a better representation of the free-solution equilibrium of the protein mixture.