Concentrations of elements in rat thymocytes measured by X-ray microanalysis

Summary Elemental concentrations of rat thymocytes in vivo were studied by X-ray microanalysis of freeze-dried sections. Cells from different regions, the subcapsular zone, the cortex and the medulla were studied in thymic tissue from a number of animals. Generally thymocytes situated in the medulla had higher concentrations of K compared to those in the subcapsular zone. The concentration of Na in the nucleus was constant in the medulla in all animals but some variation in this element was seen between animals in the subcapsular zone. The distribution of K/Na ratio in individual thymocytes was different in each region of the thymus. Cells with low K/Na ratio (<5) were predominant in the subcapsular zone, whereas cells with higher values for K/Na ratio were found in the cortex and medulla. The subcapsular zone is the region where mitotic cells are mostly situated. The finding of thymocytes with higher concentrations of Na and low K/Na ratios in this region is in accord with in vitro studies on thymocyte stimulation.