Polarographic Studies on Sarkomycin

Nicotinic acid hydrazid (NH) gave three waves at the dropping mercury electrode and can be polarographically determined. Sarkomycin nicotinic acid hydrazid derivative (SK-NH), consisting of sarkomycin (SK) and NH, gave three polarographic waves, one of which disappeared in alkaline pH region. The half-wave potential of the second wave of SK-NH was the same as that of the first wave of SK and the half-wave potential of the third wave in the acid pH region was the same as that of the second wave of NH. The relation between limiting current and concentration of SK-NH was linear, but the regression obtained by one lot of sample was different from that by another lot of sample. The regression of limiting current of the third wave at pH 8.5 upon NH content was coincident within error throughout the samples used herein.