Dipeptidyl peptidase-IV expression and activity in human glomerular endothelial cells |
| |
Authors: | Pala Laura Mannucci Edoardo Pezzatini Anna Ciani Silvia Sardi Jacopo Raimondi Laura Ognibene Agostino Cappadona Angela Vannelli Barbara G Rotella Carlo M |
| |
Affiliation: | Department of Clinical, Pathophysiology, University of Florence, Italy |
| |
Abstract: | Glucagon-like peptide-1 (GLP-1), a meal-stimulated gastrointestinal insulinotropic hormone inactivated by dipeptidyl peptidase-IV (DPP-IV), is reduced in type 2 diabetic patients. The present study shows that 2-week exposure of human glomerular endothelial cells to high glucose (22 mM) determines a highly significant increase in DPP-IV activity and mRNA expression, which cannot be entirely accounted for by hyperosmolarity. On the other hand, incubation of purified DPP-IV in a buffer solution added with high glucose does not affect enzyme activity. These results suggest that high glucose increases expression and activity of DPP-IV, possibly contributing to GLP-1 reduction in type 2 diabetic patients. |
| |
Keywords: | Glucose Peptidase GLP-1 Endothelial cells Diabetes mellitus |
本文献已被 ScienceDirect PubMed 等数据库收录! |