|
|||||
|
|
Cultured tonsillar lymphocytes excrete [3H]thymidine labeled DNA and revert to resting state |
|
| Authors: | M Sasvari-Szekely M Staub J Taljanidisz G Szabo J Takacs F Antoni |
| Institution: | 1. Membrane Bioenergetics Group, Lawrence Berkeley Laboratory, University of California, Berkeley, California 94720 USA;2. Department of Physiology and Anatomy, University of California, Berkeley, California 94720 USA |
| Abstract: | Large tonsillar lymphocytes labeled with 3H]thymidine reverted to small lymphocytes with concomitant loss of 3H]DNA upon culturing. The decrease of labeled DNA content and size of large lymphocytes was demonstrated by flow cytometry and cell sorting. These observations suggest that stimulated lymphocytes may revert from their proliferative phase to resting phase by shedding 'extra'DNA under cell culture conditions. This released DNA is not due to cell damage and can be hybridized to chromosomal DNA. |
| Keywords: | |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |