Formation and activities of xylanhydrolysing enzymes of Humicola grisea var thermoidea |
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| Authors: | B. K. Rana B. N. Johri I. S. Thakur |
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| Affiliation: | (1) Department of Environmental Sciences, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar (Nainital)-263145, U.P., India;(2) Department of Microbiology, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar (Nainital)-263145, U.P., India |
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| Abstract: | A thermophilic fungus, Humicola grisea var thermoidea, produced in liquid culture two endoxylanases (1,4- -d-xylan-xylanohydrolase, EC 3.2.1.8) with Mr of 95 (Xyl I) and 13 (Xyl II) kDa. PAGE of the crude culture filtrate and of each fraction obtained by gel filtration produced three and one band, respectively. Cross-reaction of the culture filtrate and each fraction with polyclonal antibodies prepared against Xyl II produced two and one precipitin bands, respectively. Hydrolysis of wheat straw and rice husk xylan was maximal using a combination of Xyl I and Xyl II. The products formed after hydrolysis, xylo-oligosaccharides and traces of xylose, indicated an endotype enzyme action and the co-operative activities of the xylanases. |
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| Keywords: | Co-operative activities Humicola grisea var thermoidea thermophilic fungi xylan-hydrolysing xylanases |
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