| In vitro palmitoylation of native bovine brain G_oα |
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| 作者姓名： | 杨胜于 黄有国 |
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| 作者单位： | National Laboratory of Biomacromolecule，Institute of Biophysics，Chinese Academy of Sciences，Beijing 100101，China，National Laboratory of Biomacromolecule，Institute of Biophysics，Chinese Academy of Sciences，Beijing 100101，China |
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| 基金项目： | the Chinese Academy of Sciences，中国科学院资助项目 |
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| 摘 要： | The native Goα was purified from bovine brain cortex and palmitoylated in vitro. The in vitro palmitoylation site was the same as that in vivo. The internal palmitoylation of purified native Goα was found to be largely maintained. The apparent palmitoylation ratio was significantly increased after the Goα was treated with DTT. The GTPγS binding characteristic of Goα was not influenced by palmitoylation, however, the affinity for LUVs was increased dramatically. The in vitro palmitoylation model of Goα provides a better basis for studying the functional role of G protein palmitoylation in signal transduction.
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| 关 键 词： | native Goα in vitro palmitoylation specificity GTPγS binding activity signal transduction. |
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