Structure of the O-specific polysaccharide of the bacterium Proteus mirabilis O29 |
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| Authors: | Perepelov A V Shashkov A S Senchenkova S N Knirel Y A Literacka E Kaca W |
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| Affiliation: | Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia. |
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| Abstract: | An O-specific polysaccharide was obtained by mild acid degradation of P. mirabilis O29 lipopolysaccharide (LPS) and found to contain 2-acetamido-2-deoxy-D-galactose and D-glucuronic acid (D-GlcA) in the ratio 3:1. Studies of the polysaccharide by 1H- and 13C-NMR spectroscopy including two-dimensional correlation spectroscopy (COSY), total correlation spectroscopy (TOCSY), nuclear Overhauser effect spectroscopy (NOESY), and H-detected 1H,13C-heteronuclear multiple-quantum coherence (HMQC) experiments demonstrated the following structure of the branched tetrasaccharide repeating unit: |
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