Biosynthesis of Caffeine in Flower Buds of Camellia sinensis |
| |
Authors: | Fujimori Naoko; Ashihara Hiroshi |
| |
Institution: | Department of Biology, Faculty of Science, Ochanomizu University, 2-1-1, Otsuka, Bunkyo-ku, Tokyo, 112, Japan |
| |
Abstract: | The biosynthesis of purine alkaloids in flower buds of tea plantswas investigated. More than 25% of total radioactivity of 8-14C]adeninetaken up by stamens isolated from tea flower buds was foundto have been incorporated into purine alkaloids, namely, theobromineand caffeine, 24 h after administration of the labelled compound.Pulse-chase experiments indicated that 8-14C]adenine takenup by the stamens was converted to adenine nucleotides and subsequentlyincorporated into theobromine and caffeine. Since 5 µMcoformycin, an inhibitor of AMP deaminase, inhibited the incorporationof radioactivity into the purine alkaloids, synthesis of caffeinefrom adenine nucleotides seems to be initiated by the reactionof AMP deaminase. Although most of the radioactivity from 8-14C]inosinewas recovered as CO2 and ureides, considerable amounts of radioactivitywere recovered as purine alkaloids. The incorporation of radioactivityfrom 8-14C]inosine into the purine alkaloids was not affectedby coformycin. The five enzymes involved in synthesis of 5-phosphoribosyl-1-pyrophosphatefrom glucose were present in the stamens and petals of tea flowerbuds. From present and previous results, the pathway for thebiosynthesis of caffeine from adenine nucleotides in flowerbuds of tea is discussed.Copyright 1993, 1999 Academic Press Camellia sinensis, tea, stamen, flower, biosynthesis, purine alkaloids, caffeine, theobromine, adenine nucleotides, nucleotide biosynthesis |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|