Insulin-like growth factor-I is a serum component stimulating growth of human neuroblastoma |
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Authors: | Thomas W. Burke Stanimir Vuk-Pavlović |
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Affiliation: | (1) Division of Developmental Oncology Research, Mayo Clinic and Mayo Foundation, 55905 Rochester, Minnesota |
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Abstract: | Summary Human non-autocrine neuroblastoma cells SK-N-SH and LF require serum for proliferation in vitro. We wished to determine the role of serum-borne insulin-like growth factor I (IGF-I) as mitogen for these cells. Introduction of the monoclonal antibodyα-IR3 against human IGF-I receptor reduced proliferation in the presence of fetal bovine serum (FBS). IGF-I (5 nM) was as effective as FBS (10%) in stimulating proliferation. Porcine insulin mimicked the effects of IGF-I, but at a 1000-fold higher concentration. The antibodyα-IR3 reduced growth stimulated by IGF-I more effectively than growth stimulated by insulin. Thus, proliferation of human non-autocrine neuroblastoma cells can be effectively manipulated by exogenous IGF-I. |
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Keywords: | insulin-like growth factor I insulin fetal bovine serum neuroblastoma α -IR3 antibody |
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