The photodynamic action of eosin,a singlet-oxygen generator |
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Authors: | J P Knox A D Dodge |
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Institution: | (1) School of Biological Sciences, University of Bath, BA2 7AY Bath, Avon, UK |
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Abstract: | Eosin, a known generator of singlet oxygen, applied to leaf discs of Pisum sativum L. sensitized the inhibition of photosynthesis. Analysis of partial photosynthetic electron-transport reactions and of the kinetics of variable chlorophyll fluorescence located the damage at photosystem II. This injury required the presence of oxygen and was also caused by the irradiation of eosin-treated leaf tissue with green light. The role of oxygen and photodynamic reactions in the susceptibility of photosystem II to damage by environmental stresses is discussed.Abbreviations DCMU
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- DCPIP
2,6-dichlorophenolindophenol
- DPC
1,5-diphenylcarbazide
- PSI
photosystem I
- PSII
photosystem II
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1O2
singlet oxygen
- Tricine
N-2-hydroxyl-3,1-bis(hydroxymethyl)ethyl]-glycine |
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Keywords: | Eosin (photodynamic action) Photosystem II Pisum (photosynthesis) Singlet oxygen |
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