绿色荧光蛋白与 HCV 核心蛋白的融合及在大肠杆菌中的高效表达

利用基因工程重组技术获得了绿色荧光蛋白（ｇｆｐ）基因与ＨＣＶ核心蛋白基因的嵌合体，并在大肠杆菌中高效表达了４８ｋＤａ的融合蛋白，经Ｄｏｔ－ＥＬＩＳＡ和Ｗｅｓｔｅｒｎ ｂｌｏｔ免疫活性分析证实，融合蛋白仍具有ｃｏｒｅ抗原的三个免疫活性部位，同时用荧光显微镜观察并用荧光光度计测定了大肠直菌表达的融合蛋白的荧光光谱，结果证实，我们在大肠杆菌中表达的ＧＦＰ－ｃｏｒｅ融合蛋白既能发射易于检测的绿色荧光，又具

The Fusion Expression of Green Fluorescent Protein and HCV Core Antigene in Escherichia coli Cells

A chimeric gene of the green fluorescent protein and hepatitis C virus core antigene was constructed and expressed in E.coli cells.The expressed fusion protein was examined by Dot ELISA and Western blot and the three antigenic determinants were detected.The GFP core fusion protein has not only the striking green fluorescence under natural light but also the HCV antigenic activity.A new methord of immunological diagnosis is highly expected on the light of the fusion protein which can be seen as HCV antigen tagged by equal green fluorescent protein.