Toxicity of antibiotics on zygotic embryos of white spruce (Picea glauca) cultured in vitro

The toxicity of kanamycin, hygromycin B, geneticin, methotrexate and cefotaxime on zygotic embryos ofPicea glauca was studied. Embryos placed on bud induction medium produced approximately 20 adventitious buds per embryo under control conditions. Addition of antibiotics reduced the number of bud-forming embryos. Using the percentage of embryos with buds as an indication of antibiotic toxicity, two-day-old explants were found to be more sensitive than nine-day-old. Kanamycin toxicity was enhanced by cefotaxime and this effect increased with increases in concentration of either antibiotic. Although no morphological difference was observed after 21 days, embryos growing on medium containing 20 g ml^–1 kanamycin showed a decrease of 73% in dry weight and 23% in protein content per embryo when compared to control embryos. Similarly, a decrease of 38% in dry weight and 40% in protein content per embryo was found in embryos on medium containing 300 g ml^–1 cefotaxime.Abbreviations BA 6 benzyl-aminopurine - EDTA ethylenediamine-tetraacetic acid - PVP 10 polyvinylpyrrolidone (MW 10,000) - Tris-HCl Tris [hydroxymethyl] amino hydrochlorideNRCC No. 30262